Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule
SCIE
SCOPUS
- Title
- Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule
- Authors
- Murray, James; Sim, Jaehwan; Oh, Kyunghoon; Sung, Gihyun; Lee, Ara; Shrinidhi, Annadka; Thirunarayanan, Ayyavu; Shetty, Dinesh; Kim, Kimoon
- Date Issued
- 2017-02
- Publisher
- WILEY-V C H VERLAG GMBH
- Abstract
- Chemical proteomics relies primarily on click-chemistry-based protein labeling and biotin-streptavidin enrichment, but these techniques have inherent limitations. Enrichment of intracellular proteins using a totally synthetic host-guest complex is described, overcoming the problem associated with the classical approach. We achieve this by affinity-based protein labeling with a target-specific probe molecule conjugated to a high-affinity guest (suberanilohydroxamic acid-ammonium-adamantane; SAHA-Ad) and then enriching the labeled species using a cucurbit[7]uril bead. This method shows high specificity for labeled molecules in a MDA-MB-231 breast cancer cell lysate. Moreover, this method shows promise for labeling proteins in live cells.
- Keywords
- HISTONE DEACETYLASE COMPLEXES; ACTIVITY-BASED PROBES; BIOTIN-AVIDIN PAIR; ENDOGENOUS BIOTIN; SYNTHETIC HOST; CUCURBITURIL; STREPTAVIDIN; BINDING; RECOGNITION; DERIVATIVES
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92130
- DOI
- 10.1002/anie.201611894
- ISSN
- 1433-7851
- Article Type
- Article
- Citation
- ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, vol. 56, no. 9, page. 2395 - 2398, 2017-02
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