ISOLATION OF HIGHER CROSSOVER RATE MUTANTS IN ARABIDOPSIS
- Title
- ISOLATION OF HIGHER CROSSOVER RATE MUTANTS IN ARABIDOPSIS
- Authors
- PARK, JI HYE; KIM, JUHYUN; CHOI, KYUHA; KIM, JAEIL
- Date Issued
- 2018-06-27
- Publisher
- International Conference on Arabidopsis Research
- Abstract
- Meiotic recombination produces crossovers from SPO11-mediated DSBs by diverse interhomolog
repair pathways. The crossovers affect genetic diversity in population and breeding. Crossover is
limited to ~1-3 along a chromosome by three distinct anti-crossover pathways that include FANCM,
RECQ4 and FIGL1 factors in Arabidopsis. However, the mechanism underlying crossover suppression
in which the DSBs (~40-50 per chromosome) are repaired to 1-3 crossovers remains explored clearly.
We have performed high throughput genetic screenings of higher crossover rate (hcr) mutants by using
fluorescent seed-based system, enabling the measurement of crossover frequency in individual plants
of various mutant pools. Three hcr mutants (hcr1, hcr2, hcr3) were isolated and confirmed to be new
anti-crossover mutants by genetic analyses and crossover measurements. The isolation and
characterization of hcr mutants reveal new anti-crossover pathways, providing an insight into the
crossover suppression mechanism in plants.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/92260
- Article Type
- Conference
- Citation
- 29th International Conference on Arabidopsis Research 2018, page. 427 - 427, 2018-06-27
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