DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, D | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | Ahn, MM | - |
dc.contributor.author | Kang, IS | - |
dc.contributor.author | Park, KH | - |
dc.contributor.author | Jeon, S | - |
dc.date.accessioned | 2018-10-04T05:51:31Z | - |
dc.date.available | 2018-10-04T05:51:31Z | - |
dc.date.created | 2016-02-19 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/92373 | - |
dc.description.abstract | A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | ANALYTICA CHIMICA ACTA | - |
dc.title | Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/J.ACA.2015.04.044 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, v.883, pp.61 - 66 | - |
dc.identifier.wosid | 000356236700007 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 66 | - |
dc.citation.startPage | 61 | - |
dc.citation.title | ANALYTICA CHIMICA ACTA | - |
dc.citation.volume | 883 | - |
dc.contributor.affiliatedAuthor | Ahn, MM | - |
dc.contributor.affiliatedAuthor | Kang, IS | - |
dc.contributor.affiliatedAuthor | Jeon, S | - |
dc.identifier.scopusid | 2-s2.0-84931006192 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 22 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | HYDROGEN-PEROXIDE SENSOR | - |
dc.subject.keywordPlus | SINGLE CARBON-FIBER | - |
dc.subject.keywordPlus | TITANIUM-DIOXIDE | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | CAPACITORS | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | NANORODS | - |
dc.subject.keywordPlus | CATALYST | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordAuthor | ruthenium oxide | - |
dc.subject.keywordAuthor | titanium oxide | - |
dc.subject.keywordAuthor | nanoneedle | - |
dc.subject.keywordAuthor | nanofiber | - |
dc.subject.keywordAuthor | electrocatalyst | - |
dc.subject.keywordAuthor | H2O2 electrochemical reaction | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
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