DC Field | Value | Language |
---|---|---|
dc.contributor.author | Garcia, NV | - |
dc.contributor.author | Umemoto, E | - |
dc.contributor.author | Saito, Y | - |
dc.contributor.author | Yamasaki, M | - |
dc.contributor.author | Hata, E | - |
dc.contributor.author | Matozaki, T | - |
dc.contributor.author | Murakami, M | - |
dc.contributor.author | Jung, YJ | - |
dc.contributor.author | Woo, SY | - |
dc.contributor.author | Seoh, JY | - |
dc.contributor.author | Jang, MH | - |
dc.contributor.author | Aozasa, K | - |
dc.contributor.author | Miyasaka, M | - |
dc.date.accessioned | 2018-10-04T05:57:58Z | - |
dc.date.available | 2018-10-04T05:57:58Z | - |
dc.date.created | 2011-08-05 | - |
dc.date.issued | 2011-09-01 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/92455 | - |
dc.description.abstract | Eosinophils are abundant in the lamina propria of the small intestine, but they rarely show degranulation in situ under steady-state conditions. In this study, using two novel mAbs, we found that intestinal eosinophils constitutively expressed a high level of an inhibitory receptor signal regulatory protein alpha (SIRP alpha)/CD172a and a low, but significant, level of a tetraspanin CD63, whose upregulation is closely associated with degranulation. Cross-linking SIRP alpha/CD172a on the surface of wild-type eosinophils significantly inhibited the release of eosinophil peroxidase induced by the calcium ionophore A23187, whereas this cross-linking effect was not observed in eosinophils isolated from mice expressing a mutated SIRP alpha/CD172a that lacks most of its cytoplasmic domain (SIRP alpha Cyto(-/-)). The SIRP alpha Cyto(-/-) eosinophils showed reduced viability, increased CD63 expression, and increased eosinophil peroxidase release with or without A23187 stimulation in vitro. In addition, SIRP alpha Cyto(-/-) mice showed increased frequencies of Annexin V-binding eosinophils and free MBP(+)CD63(+) extracellular granules, as well as increased tissue remodeling in the small intestine under steady-state conditions. Mice deficient in CD47, which is a ligand for SIRP alpha/CD172a, recapitulated these phenomena. Moreover, during Th2-biased inflammation, increased eosinophil cell death and degranulation were obvious in a number of tissues, including the small intestine, in the SIRP alpha Cyto(-/-) mice compared with wild-type mice. Collectively, our results indicated that SIRP alpha/CD172a regulates eosinophil homeostasis, probably by interacting with CD47, with substantial effects on eosinophil survival. Thus, SIRP alpha/CD172a is a potential therapeutic target for eosinophil-associated diseases. The Journal of Immunology, 2011, 187: 2268-2277. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.relation.isPartOf | JOURNAL OF IMMUNOLOGY | - |
dc.title | SIRP alpha/CD172a Regulates Eosinophil Homeostasis | - |
dc.type | Article | - |
dc.identifier.doi | 10.4049/JIMMUNOL.1101008 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.187, no.5, pp.2268 - 2277 | - |
dc.identifier.wosid | 000294059500031 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 2277 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 2268 | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 187 | - |
dc.contributor.affiliatedAuthor | Jang, MH | - |
dc.identifier.scopusid | 2-s2.0-80052688904 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 25 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | INTEGRIN-ASSOCIATED PROTEIN | - |
dc.subject.keywordPlus | ACTIVATED HUMAN EOSINOPHILS | - |
dc.subject.keywordPlus | INNATE IMMUNE-SYSTEM | - |
dc.subject.keywordPlus | MAJOR BASIC-PROTEIN | - |
dc.subject.keywordPlus | PEROXIDASE RELEASE | - |
dc.subject.keywordPlus | SIRP-ALPHA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MEMBRANE GLYCOPROTEIN | - |
dc.subject.keywordPlus | ULCERATIVE-COLITIS | - |
dc.subject.keywordPlus | TYPE-2 IMMUNITY | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
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