음식물류폐기물 재활용공정 폐수내 입자성 물질의 혐기성 가수분해 및 산생성미생물 군집구조

Abstract

The purpose of this study was to investigate the relationships between hydrolysis of particulate organic materials (POM) and microorganisms in acidogenic reactors treating Food-waste recycling wastewater (FRW). Hydrolysis efficiency in terms of volatile suspended solid (VSS) reduction was observed in continuous completely stirred tank reactors (CSTRs) with changing hydraulic retention time HRT (1 ― 3 day), pH (4 ― 6), and temperature (25 ― 45oC) and was interpreted by response surface methodology (RSM). Denaturing gel gradient electrophoresis (DGGE) using bacterial 16S rRNA genes were also carried out to investigate acidogenic bacterial community structure. The ten identified acidogenic bacterial microorganisms (ABMs) were selected as target for developing species-specific primers and probe set for use of real time quantitative polymerase chain reaction (QPCR). Each ABM in the acidogenic reactor treating FRW was successfully quantified by using designed primers and probe set. Redundancy analysis (RDA) elucidated the relationships between 16S rRNA gene concentration of ABMs and hydrolysis efficiency.