SEQUENCING OF SPO11-1-OLIGONUCLEOTIDES IN H2A.Z DEPOSITION MUTANT IN ARABIDOPSIS
- Title
- SEQUENCING OF SPO11-1-OLIGONUCLEOTIDES IN H2A.Z DEPOSITION MUTANT IN ARABIDOPSIS
- Authors
- CHOI, KYUHA; PARK, JI HYE; KIM, JUHYUN; KIM, JAE IL
- Date Issued
- 2018-06-27
- Publisher
- International Conference on Arabidopsis Research
- Abstract
- Meiotic recombination is initiated by a widely conserved SPO11 transesterase-mediated DSBs. The meiotic DSB
landscapes vary among species and depend on either specific DNA sequence or chromatin structure. By
sequencing Arabidopsis SPO11-1-oligonucleitides we found that meiotic DSB frequency and distribution are
quantitatively correlated with chromatin accessibility. Consistently, DNA methylation and H3K9 dimthylation
inhibit meiotic DSB formation around hetetochromatic pericentromeres and centromeres. However, how
chromatin remodeling factors including SWR1-complex influence the meiotic DSBs remain elusive in Arabidopsis.
We mapped a high-profile DSB landscape in arp6 mutant, defective in H2A.Z deposition. The ATP-dependent
SWR1 chromatin remodeling shapes meiotic DSB pattern around gene promoters and terminators. Our results
provide an insight into how meiotic recombination initiation is finely controlled by SWR1 chromatin remodeling
complex.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94034
- Article Type
- Conference
- Citation
- 29th International Conference on Arabidopsis Research (ICAR 2018), page. 81 - 81, 2018-06-27
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