DC Field | Value | Language |
---|---|---|
dc.contributor.author | KIM, TAE KYUNG | - |
dc.date.accessioned | 2018-12-04T01:54:47Z | - |
dc.date.available | 2018-12-04T01:54:47Z | - |
dc.date.created | 2018-11-21 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 0076-6879 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/94313 | - |
dc.description.abstract | Genome-wide analyses have revolutionized our ability to study the transcriptional regulation of circadian rhythms. The advent of next-generation sequencing methods has facilitated the use of two such technologies, ChIP-seq and RNA-seq. In this chapter, we describe detailed methods and protocols for these two techniques, with emphasis on their usage in circadian rhythm experiments in the mouse liver, a major target organ of the circadian clock system. Critical factors for these methods are highlighted and issues arising with time series samples for ChIP-seq and RNA-seq are discussed. Finally, detailed protocols for library preparation suitable for Illumina sequencing platforms are presented. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ACADEMIC PRESS INC | - |
dc.relation.isPartOf | METHODS IN ENZYMOLOGY | - |
dc.title | ChIP-seq and RNA-seq methods to study circadian control of transcription in mammals. | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/bs.mie.2014.10.059 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | METHODS IN ENZYMOLOGY, v.551, pp.285 - 321 | - |
dc.identifier.wosid | 000405614400015 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 321 | - |
dc.citation.startPage | 285 | - |
dc.citation.title | METHODS IN ENZYMOLOGY | - |
dc.citation.volume | 551 | - |
dc.contributor.affiliatedAuthor | KIM, TAE KYUNG | - |
dc.identifier.scopusid | 2-s2.0-84961379599 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 7 | - |
dc.type.docType | Review; Book Chapter | - |
dc.subject.keywordPlus | CROSS-LINKING | - |
dc.subject.keywordPlus | CHROMATIN IMMUNOPRECIPITATION | - |
dc.subject.keywordPlus | DNA-STRUCTURE | - |
dc.subject.keywordPlus | CLOCK | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | FORMALDEHYDE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | POLYMERASE | - |
dc.subject.keywordPlus | MOUSE | - |
dc.subject.keywordPlus | PROBE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Physiology | - |
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