Microfabrication of In Vitro Alveolar-Capillary Barrier Model by Inkjet-based Bioprinting
- Title
- Microfabrication of In Vitro Alveolar-Capillary Barrier Model by Inkjet-based Bioprinting
- Authors
- JUNG, SUNGJUNE
- Date Issued
- 2018-09-05
- Publisher
- Tissue Engineering and Regenerative Medicine International Society
- Abstract
- An in vitro alveolar-capillary barrier is one of the essential model systems for pulmonary drug and particle tests in disease studies, drug
discovery and toxicology. An alveolar-capillary barrier in the gas exchanging region of the lung consists of epithelial and endothelial layers with
a thickness of 2 μm. This thin structure is critical to sustain pulmonary function such as gas diffusion. There has been efforts to fabricate the
biomimetic human alveolar-capillary barrier model, using microfluidic devices and bioprinting technology. However, none of the works has
achieved to mimic this thin membrane, a key feature for the model. Here, we present a human alveolar-capillary model with a sub-10 mm-thick
membrane, containing multi-type alveolar cells. We fabricated the alveolar-capillary barrier model with four types of human alveolar cell lines,
including type 1 alveolar cell (NCI-H1703), type 2 alveolar cell (A549), lung fibroblast (MRC5), and lung microvascular endothelial cell (HULEC5a).
High-resolution drop-on-demand inkjet printing enabled the fabrication of the thin alveolar-capillary barrier model under sub-10 mm
thickness for the optimal structure by drop-on-demand deposition of multi-type alveolar cells as a thin layer. We evaluated the functions of the
fabricated models by histology, barrier integrity test, and barrier permeability test to demonstrate the level of biomimicry. Inkjet-based
bioprinting enabled the fabrication of reproducible in vitro alveolar-capillary models, which have biomimetic microstructures with customized
and functionally designed micro-patterns. The inkjet-bioprinted alveolar-capillary models have a potential to replace animal testing as
expecting to be applied in disease models for pathology, drug discovery, and toxicology
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94774
- Article Type
- Conference
- Citation
- 5th TERMIS World Congress 2018, 2018-09-05
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