Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins
SCIE
SCOPUS
- Title
- Efficient and wash-free labeling of membrane proteins using engineered Npu DnaE split-inteins
- Authors
- Lee, Euiyeon; Min, Kyoungmi; Chang, Young-Tae; Kwon, Youngeun
- Date Issued
- 2018-09
- Publisher
- WILEY
- Abstract
- An efficient and wash-free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split-inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific binding and covalent conjugation, are observed. The covalent labeling was monitored within 5 min, without background staining.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/95655
- DOI
- 10.1002/pro.3455
- ISSN
- 0961-8368
- Article Type
- Article
- Citation
- PROTEIN SCIENCE, vol. 27, no. 9, page. 1568 - 1574, 2018-09
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- There are no files associated with this item.
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