Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication
SCIE
SCOPUS
- Title
- Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication
- Authors
- Han, Julianna; Perez, Jasmine T.; Chen, Cindy; Li, Yan; Benitez, Asiel; Kandasamy, Matheswaran; Lee, Yoontae; Andrade, Jorge; tenoever, Benjamin; Manicassamy, Balaji
- Date Issued
- 2018-04
- Publisher
- Cell Press
- Abstract
- The emergence of influenza A viruses (IAVs) from zoonotic reservoirs poses a great threat to human health. As seasonal vaccines are ineffective against zoonotic strains, and newly transmitted viruses can quickly acquire drug resistance, there remains a need for host-directed therapeutics against IAVs. Here, we performed a genome-scale CRISPR/Cas9 knockout screen in human lung epithelial cells with a human isolate of an avian H5N1 strain. Several genes involved in sialic acid biosynthesis and related glycosylation pathways were highly enriched post-H5N1 selection, including SLC35A1, a sialic acid transporter essential for IAV receptor expression and thus viral entry. Importantly, we have identified capicua (CIC) as a negative regulator of cell-intrinsic immunity, as loss of CIC resulted in heightened antiviral responses and restricted replication of multiple viruses. Therefore, our study demonstrates that the CRISPR/Cas9 system can be utilized for the discovery of host factors critical for the replication of intracellular pathogens.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/99297
- DOI
- 10.1016/j.celrep.2018.03.045
- ISSN
- 2211-1247
- Article Type
- Article
- Citation
- Cell Reports, vol. 23, no. 2, page. 596 - 607, 2018-04
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