Real-time detection and classification of cells in a fluid flow

Abstract

Flow cytometry is a method of measuring the intensity of the fluorescence produced by labeled cells. However, the fluorescence intensity does not provide any spatial information and the equipment is expensive. The present study examined a real-time system for detecting and classifying cells in a fluid flow that was based on a low-cost CCD camera. To investigate the feasibility of the system, processing time measurements and image classification were conducted.