DC Field | Value | Language |
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dc.contributor.author | Kim, Dong Sung | - |
dc.contributor.author | Kim, Hong Kyun | - |
dc.contributor.author | Park, Byeong-ung | - |
dc.contributor.author | Park, Sang Min | - |
dc.contributor.author | Lee, Kyoung-pil | - |
dc.contributor.author | Lee, Seong Jin | - |
dc.contributor.author | Nam, Yu Eun | - |
dc.contributor.author | Park, Han Sang | - |
dc.contributor.author | Eom, Seongsu | - |
dc.contributor.author | Lim, Jeong Ok | - |
dc.date.accessioned | 2019-12-06T08:50:03Z | - |
dc.date.available | 2019-12-06T08:50:03Z | - |
dc.date.created | 2019-11-20 | - |
dc.date.issued | 2019-11 | - |
dc.identifier.issn | 2041-7314 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/100452 | - |
dc.description.abstract | The endothelialization on the poly (epsilon-caprolactone) nanofiber has been limited due to its low hydrophilicity. The aim of this study was to immobilize collagen on an ultra-thin poly (epsilon-caprolactone) nanofiber membrane without altering the nanofiber structure and maintaining the endothelial cell homeostasis on it. We immobilized collagen on the poly (epsilon-caprolactone) nanofiber using hydrolysis by NaOH treatment and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo-N-hydroxysulfosuccinimide reaction as a cost-effective and stable approach. NaOH was first applied to render the poly (epsilon-caprolactone) nanofiber hydrophilic. Subsequently, collagen was immobilized on the surface of the poly (epsilon-caprolactone) nanofibers using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/sulfo-N-hydroxysulfosuccinimide. Scanning electron microscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and fluorescence microscopy were used to verify stable collagen immobilization on the surface of the poly (epsilon-caprolactone) nanofibers and the maintenance of the original structure of poly (epsilon-caprolactone) nanofibers. Furthermore, human endothelial cells were cultured on the collagen-immobilized poly (epsilon-caprolactone) nanofiber membrane and expressed tight junction proteins with the increase in transendothelial electrical resistance, which demonstrated the maintenance of the endothelial cell homeostasis on the collagen-immobilized-poly (epsilon-caprolactone) nanofiber membrane. Thus, we expected that this process would be promising for maintaining cell homeostasis on the ultra-thin poly (epsilon-caprolactone) nanofiber scaffolds. | - |
dc.language | English | - |
dc.publisher | SAGE-Hindawi Access to Research | - |
dc.relation.isPartOf | Journal of Tissue Engineering | - |
dc.title | Collagen Immobilization on Ultra-thin Nanofiber Membrane to Promote In Vitro Endothelial Monolayer Formation | - |
dc.type | Article | - |
dc.identifier.doi | 10.1177/2041731419887833 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Journal of Tissue Engineering, v.10, pp.1 - 12 | - |
dc.identifier.wosid | 000497652900001 | - |
dc.citation.endPage | 12 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | Journal of Tissue Engineering | - |
dc.citation.volume | 10 | - |
dc.contributor.affiliatedAuthor | Kim, Dong Sung | - |
dc.contributor.affiliatedAuthor | Park, Sang Min | - |
dc.contributor.affiliatedAuthor | Lee, Seong Jin | - |
dc.contributor.affiliatedAuthor | Eom, Seongsu | - |
dc.identifier.scopusid | 2-s2.0-85075124907 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.type.docType | ARTICLE | - |
dc.subject.keywordPlus | Tissue engineering | - |
dc.subject.keywordPlus | nanofiber membrane | - |
dc.subject.keywordPlus | collagen | - |
dc.subject.keywordPlus | biomimetic model | - |
dc.subject.keywordPlus | post-processing | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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