Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound
- Title
- Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound
- Authors
- LEE, Jae-Jung; Kim, Jun-Young; Zhai, Duanting; Yun, Seong-Wook; Chang, Young-Tae
- Date Issued
- 2012-06
- Publisher
- KISTI
- Abstract
- Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Westernblotting
of proteins of interest especially when specific antibodies for the proteins are
not available. However, the conventional Western-blotting requires the multi-steps
process taking at least several hours up to two days. With examples of various applications,
here we show a convenient and time-saving method for protein detection which
employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose
“Estern staining”, as a standard term for protein detection method using fluorescent
chemicals and their binding small peptide tags. Eastern staining may substitutes for the
time-consuming “immuno-staining” in many versatile applications.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/100521
- DOI
- 10.4051 / ibc.2012.4.2.0005
- ISSN
- 2005-8543
- Article Type
- Article
- Citation
- Interdisciplinary Bio Central, vol. 4, no. 5, page. 1 - 9, 2012-06
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