Validation of CDr15 as a new dye for detecting neutrophil extracellular trap
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SCOPUS
- Title
- Validation of CDr15 as a new dye for detecting neutrophil extracellular trap
- Authors
- Kim, Sang-Jin; Kim, Jiyoung; Kim, Bonah; Lee, Won-Woo; Liu, Xiao; Chang, Young-Tae; Park, Jong-Wan
- Date Issued
- 2020-06
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Abstract
- Neutrophil extracellular trap (NET) is one of the first-line defenses against microbes. Under certain circumstances, however, it also plays an aggravating factor in diverse inflammation-related diseases including cancers and vascular diseases. Our aim is to develop a new method to detect NET in cells and tissues using a DNA-specific fluorescence probe CDr15. CDr15 was characterized to be impermeable to the cell membranes and to emit a strong fluorescence in association with extracellular DNAs in NET. Due to these properties, CDr15 was successfully shown to quantify NETs in vitro and to be applicable for real-time monitoring NET formation in PMA-stimulated neutrophils. Even in formaldehyde-fixed tumor specimens, CDr15 could detect NETs spreading around cancer cells. Compared with DAPI and SYTOX DNA dyes, CDr15 showed a lower level of background fluorescence and a higher specificity in NET detection. Based on these results, we propose CDr15 as a novel marker of NET to be applicable in experimental and clinical studies. (C) 2020 Elsevier Inc. All rights reserved.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/103867
- DOI
- 10.1016/j.bbrc.2020.04.153
- ISSN
- 0006-291X
- Article Type
- Article
- Citation
- BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 527, no. 3, page. 646 - 653, 2020-06
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