DC Field | Value | Language |
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dc.contributor.author | SHIM, WOO CHEOL | - |
dc.contributor.author | WOO, SUNGWOOK | - |
dc.contributor.author | PARK, JOON WON | - |
dc.date.accessioned | 2022-10-04T11:00:06Z | - |
dc.date.available | 2022-10-04T11:00:06Z | - |
dc.date.created | 2022-07-19 | - |
dc.date.issued | 2022-02 | - |
dc.identifier.issn | 1530-6984 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/113885 | - |
dc.description.abstract | Methylation changes at cytosine-guanine dinucleotide (CpG) sites in genes are closely related to cancer development. Thus, detection and quantification of low-abundance methylated DNA is critical for early diagnosis. Here, we report an atomic force microscopy (AFM)-based quantification method for DNA that contains methyl-CpG at a specific site, without any treatment to the target DNA such as chemical labeling, fluorescence tagging, or amplification. We employed AFM-tip-tethered methyl-CpG-binding proteins to probe surface-captured methylated DNA. We observed a linear correlation (R-2 = 0.982) between the input copy number and detected copy number, in the low copy number regime (10 or fewer; subattomolar concentrations). For a mixture of methylated and nonmethylated DNA that resembles clinical samples, we were still able to quantify the methylated DNA. These results highlight the potential of our force-mapping-based quantification method for wide applications in early detection of diseases associated with methylated DNA. | - |
dc.language | English | - |
dc.publisher | American Chemical Society | - |
dc.relation.isPartOf | Nano Letters | - |
dc.title | Nanoscale Force-mapping based quantification of low-abundance methylated DNA | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.nanolett.1c04637 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | Nano Letters, v.22, no.3, pp.1324 - 1330 | - |
dc.identifier.wosid | 000777171600059 | - |
dc.citation.endPage | 1330 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1324 | - |
dc.citation.title | Nano Letters | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | SHIM, WOO CHEOL | - |
dc.contributor.affiliatedAuthor | WOO, SUNGWOOK | - |
dc.contributor.affiliatedAuthor | PARK, JOON WON | - |
dc.identifier.scopusid | 2-s2.0-85124120841 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | CPG-BINDING DOMAIN | - |
dc.subject.keywordPlus | RESTRICTION ENZYMES | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | HYPERMETHYLATION | - |
dc.subject.keywordPlus | LOCALIZATION | - |
dc.subject.keywordPlus | SURFACE | - |
dc.subject.keywordPlus | ENERGY | - |
dc.subject.keywordPlus | SITES | - |
dc.subject.keywordAuthor | atomic force microscopy | - |
dc.subject.keywordAuthor | single-molecule force spectroscopy | - |
dc.subject.keywordAuthor | cytosine-guanine dinucleotide | - |
dc.subject.keywordAuthor | 5-methylcytosine | - |
dc.subject.keywordAuthor | methyl-CpG-binding domain protein | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
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