Nanoscale Force-mapping based quantification of low-abundance methylated DNA
SCIE
SCOPUS
- Title
- Nanoscale Force-mapping based quantification of low-abundance methylated DNA
- Authors
- SHIM, WOO CHEOL; WOO, SUNGWOOK; PARK, JOON WON
- Date Issued
- 2022-02
- Publisher
- American Chemical Society
- Abstract
- Methylation changes at cytosine-guanine dinucleotide (CpG) sites in genes are closely related to cancer development. Thus, detection and quantification of low-abundance methylated DNA is critical for early diagnosis. Here, we report an atomic force microscopy (AFM)-based quantification method for DNA that contains methyl-CpG at a specific site, without any treatment to the target DNA such as chemical labeling, fluorescence tagging, or amplification. We employed AFM-tip-tethered methyl-CpG-binding proteins to probe surface-captured methylated DNA. We observed a linear correlation (R-2 = 0.982) between the input copy number and detected copy number, in the low copy number regime (10 or fewer; subattomolar concentrations). For a mixture of methylated and nonmethylated DNA that resembles clinical samples, we were still able to quantify the methylated DNA. These results highlight the potential of our force-mapping-based quantification method for wide applications in early detection of diseases associated with methylated DNA.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/113885
- DOI
- 10.1021/acs.nanolett.1c04637
- ISSN
- 1530-6984
- Article Type
- Article
- Citation
- Nano Letters, vol. 22, no. 3, page. 1324 - 1330, 2022-02
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.