SARS-CoV-2 spike trimer vaccine expressed in Nicotiana benthamiana adjuvanted with Alum elicits protective immune responses in mice
SCIE
SCOPUS
- Title
- SARS-CoV-2 spike trimer vaccine expressed in Nicotiana benthamiana adjuvanted with Alum elicits protective immune responses in mice
- Authors
- Song, Shi‐Jian; Kim, Heeyeon; Jang, Eun Young; Jeon, Hyungmin; Diao, Hai‐Ping; Khan, Md Rezaul Islam; Lee, Mi‐Seon; Lee, Young Jae; Nam, Jeong‐hyun; Kim, Seong‐Ryeol; Kim, Young‐Jin; Sohn, Eun‐Ju; Hwang, Inhwan; Choi, Jang‐Hoon
- Date Issued
- 2022-12
- Publisher
- Blackwell Publishing Inc.
- Abstract
- The ongoing coronavirus disease 2019 (COVID-19) pandemic has spurred rapid development of vaccines as part of the public health response. However, the general strategy used to construct recombinant trimeric severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) proteins in mammalian cells is not completely adaptive to molecular farming. Therefore, we generated several constructs of recombinant S proteins for high expression in Nicotiana benthamiana. Intramuscular injection of N. benthamiana-expressed S-ct vaccine (NS(ct)Vac) into Balb/c mice elicited both humoral and cellular immune responses, and booster doses increased neutralizing antibody titres. In human angiotensin-converting enzyme knock-in mice, two doses of NS(ct)Vac induced anti-S and neutralizing antibodies, which cross-neutralized Alpha, Beta, Delta and Omicron variants. Survival rates after lethal challenge with SARS-CoV-2 were up to 80%, without significant body weight loss, and viral titres in lung tissue fell rapidly, with no infectious virus detectable at 7-day post-infection. Thus, plant-derived NS(ct)Vac could be a candidate COVID-19 vaccine.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/116301
- DOI
- 10.1111/pbi.13908
- ISSN
- 1467-7644
- Article Type
- Article
- Citation
- Plant Biotechnology Journal, vol. 20, no. 12, page. 2298 - 2312, 2022-12
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- There are no files associated with this item.
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