A blend of broadly-reactive and pathogen-selected Vγ4 Vδ1 T cell receptors confer broad bacterial reactivity of resident memory γδ T cells
SCIE
SCOPUS
- Title
- A blend of broadly-reactive and pathogen-selected Vγ4 Vδ1 T cell receptors confer broad bacterial reactivity of resident memory γδ T cells
- Authors
- Khairallah, Camille; Bettke, Julie A.; Gorbatsevych, Oleksandr; Qiu, Zhijuan; Zhang, Yue; Cho, Kyungjin; Kim, Kwang Soon; Chu, Timothy H.; Imperato, Jessica N.; Hatano, Shinya; Romanov, Galina; Yoshikai, Yasunobo; Puddington, Lynn; Surh, Charles D.; Bliska, James B.; van der Velden, Adrianus W.M.; Sheridan, Brian S.
- Date Issued
- 2022-01
- Publisher
- Nature Publishing Group
- Abstract
- Although murine γδ T cells are largely considered innate immune cells, they have recently been reported to form long-lived memory populations. Much remains unknown about the biology and specificity of memory γδ T cells. Here, we interrogated intestinal memory Vγ4 Vδ1 T cells generated after foodborne Listeria monocytogenes (Lm) infection to uncover an unanticipated complexity in the specificity of these cells. Deep TCR sequencing revealed that a subset of non-canonical Vδ1 clones are selected by Lm infection, consistent with antigen-specific clonal expansion. Ex vivo stimulations and in vivo heterologous challenge infections with diverse pathogenic bacteria revealed that Lm-elicited memory Vγ4 Vδ1 T cells are broadly reactive. The Vγ4 Vδ1 T cell recall response to Lm, Salmonella enterica serovar Typhimurium (STm) and Citrobacter rodentium was largely mediated by the γδTCR as internalizing the γδTCR prevented T cell expansion. Both broadly-reactive canonical and pathogen-selected non-canonical Vδ1 clones contributed to memory responses to Lm and STm. Interestingly, some non-canonical γδ T cell clones selected by Lm infection also responded after STm infection, suggesting some level of cross-reactivity. These findings underscore the promiscuous nature of memory γδ T cells and suggest that pathogen-elicited memory γδ T cells are potential targets for broad-spectrum anti-infective vaccines. © 2021, The Author(s), under exclusive licence to Society for Mucosal Immunology.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/118936
- DOI
- 10.1038/s41385-021-00447-x
- ISSN
- 1933-0219
- Article Type
- Article
- Citation
- Mucosal Immunology, vol. 15, no. 1, page. 176 - 187, 2022-01
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