Laminin-Augmented Decellularized Extracellular Matrix Ameliorating Neural Differentiation and Neuroinflammation in Human Mini-Brains
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SCOPUS
- Title
- Laminin-Augmented Decellularized Extracellular Matrix Ameliorating Neural Differentiation and Neuroinflammation in Human Mini-Brains
- Authors
- BAE, MIHYEON; Huyen Ngo; You Jung Kang; Su-Jin Lee; PARK, WONBIN; JO, YEONGGWON; CHOI, YOO MI; KIM, JOENGJU; Hee-Gyeong Yi; Hyung-Seok Kim; JANG, JIN AH; CHO, DONGWOO; Hansang Cho
- Date Issued
- 2023-12
- Publisher
- Wiley - V C H Verlag GmbbH & Co.
- Abstract
- Non-neural extracellular matrix (ECM) has limited application in humanized physiological neural modeling due to insufficient brain-specificity and safety concerns. Although brain-derived ECM contains enriched neural components, certain essential components are partially lost during the decellularization process, necessitating augmentation. Here, it is demonstrated that the laminin-augmented porcine brain-decellularized ECM (P-BdECM) is xenogeneic factor-depleted as well as favorable for the regulation of human neurons, astrocytes, and microglia. P-BdECM composition is comparable to human BdECM regarding brain-specificity through the matrisome and gene ontology-biological process analysis. As augmenting strategy, laminin 111 supplement promotes neural function by synergic effect with laminin 521 in P-BdECM. Annexin A1(ANXA1) and Peroxiredoxin(PRDX) in P-BdECM stabilized microglial and astrocytic behavior under normal while promoting active neuroinflammation in response to neuropathological factors. Further, supplementation of the brain-specific molecule to non-neural matrix also ameliorated glial cell inflammation as in P-BdECM. In conclusion, P-BdECM-augmentation strategy can be used to recapitulate humanized pathophysiological cerebral environments for neurological study.
Porcine-derived brain decellularized extracellular matrix (P-BdECM) augmented with LN111 is developed to construct physiological human brain model. The similarity of P-BdECM is specifically validated to human BdECM and brain-specific proteins in ameliorating neural differentiation and neuroinflammation. The augmented P-BdECM is favorable to recapitulate the neural microenvironment for the study of cellular behavior and the role of ECM in neural processes.image
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/120687
- DOI
- 10.1002/smll.202308815
- ISSN
- 1613-6810
- Article Type
- Article
- Citation
- Small, vol. 20, no. 23, 2023-12
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- There are no files associated with this item.
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