ChIP-Seq Strategy to Identify Z-DNA-Forming Sequences in the Human Genome
SCOPUS
- Title
- ChIP-Seq Strategy to Identify Z-DNA-Forming Sequences in the Human Genome
- Authors
- Roh, Tae-Young
- Date Issued
- 2023-01
- Publisher
- Humana Press Inc.
- Abstract
- Different from the canonical right-handed B-DNA, a left-handed Z-DNA forms an alternating syn- and anti-base conformations along the double-stranded helix under physiological conditions. Z-DNA structure plays a role in transcriptional regulation, chromatin remodeling, and genome stability. To understand the biological function of Z-DNA and map the genome-wide Z-DNA-forming sites (ZFSs), a ChIP-Seq strategy is applied, which is a combination of chromatin immunoprecipitation (ChIP) and high-throughput DNA sequencing analysis. Cross-linked chromatin is sheared and its fragments associated with Z-DNA-binding proteins are mapped onto the reference genome sequence. The global information of ZFSs positioning can provide a useful resource for better understanding of DNA structure-dependent biological mechanism.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/123703
- DOI
- 10.1007/978-1-0716-3084-6_12
- ISSN
- 1064-3745
- Article Type
- Article
- Citation
- Methods in Molecular Biology, vol. 2651, page. 167 - 177, 2023-01
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- There are no files associated with this item.
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