Centrifugal Multiplexing Fixed-Volume Dispenser on a Plastic Lab-on-a-Disk for Parallel Biochemical Single-End-Point Assays
SCIE
SCOPUS
- Title
- Centrifugal Multiplexing Fixed-Volume Dispenser on a Plastic Lab-on-a-Disk for Parallel Biochemical Single-End-Point Assays
- Authors
- Moonwoo La; Sang Min Park; Kim, DS
- Date Issued
- 2015-01
- Publisher
- AMER INST PHYSICS
- Abstract
- In this study, a multiple sample dispenser for precisely metered fixed volumes was successfully designed, fabricated, and fully characterized on a plastic centrifugal lab-on-a-disk (LOD) for parallel biochemical single-end-point assays. The dispenser, namely, a centrifugal multiplexing fixed-volume dispenser (C-MUFID) was designed with microfluidic structures based on the theoretical modeling about a centrifugal circumferential filling flow. The designed LODs were fabricated with a polystyrene substrate through micromachining and they were thermally bonded with a flat substrate. Furthermore, six parallel metering and dispensing assays were conducted at the same fixed-volume (1.27 mu l) with a relative variation of +/- 0.02 mu l. Moreover, the samples were metered and dispensed at different sub-volumes. To visualize the metering and dispensing performances, the C-MUFID was integrated with a serpentine micromixer during parallel centrifugal mixing tests. Parallel biochemical single-end-point assays were successfully conducted on the developed LOD using a standard serum with albumin, glucose, and total protein reagents. The developed LOD could be widely applied to various biochemical single-end-point assays which require different volume ratios of the sample and reagent by controlling the design of the C-MUFID. The proposed LOD is feasible for point-of-care diagnostics because of its mass-producible structures, reliable metering/dispensing performance, and parallel biochemical single-end-point assays, which can identify numerous biochemical. (c) 2015 AIP Publishing LLC.
- Keywords
- FILLING FLOW; MICROCHANNEL; DEVICE; FORCE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/13074
- DOI
- 10.1063/1.4905940
- ISSN
- 1932-1058
- Article Type
- Article
- Citation
- BIOMICROFLUIDICS, vol. 9, no. 1, page. 14104, 2015-01
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