DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chang, M | - |
dc.contributor.author | Kwon, M | - |
dc.contributor.author | Kim, S | - |
dc.contributor.author | Yunn, NO | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | LEE, JONG BONG | - |
dc.date.accessioned | 2016-03-31T08:13:44Z | - |
dc.date.available | 2016-03-31T08:13:44Z | - |
dc.date.created | 2014-03-06 | - |
dc.date.issued | 2014-05 | - |
dc.identifier.issn | 1083-3668 | - |
dc.identifier.other | 2014-OAK-0000029183 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/14842 | - |
dc.description.abstract | We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-beta-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | SPIE | - |
dc.relation.isPartOf | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.title | Aptamer-based single-molecule imaging of insulin receptors in living cells | - |
dc.type | Article | - |
dc.contributor.college | 물리학과 | - |
dc.identifier.doi | 10.1117/1.JBO.19.5.051204 | - |
dc.author.google | Chang M., Kwon M., Kim S., Yunn N.-O., Kim D., Ryu S.H., Lee J.-B. | - |
dc.relation.volume | 19 | - |
dc.relation.issue | 5 | - |
dc.contributor.id | 10171158 | - |
dc.relation.journal | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOMEDICAL OPTICS, v.19, no.5 | - |
dc.identifier.wosid | 000338334600005 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.number | 5 | - |
dc.citation.title | JOURNAL OF BIOMEDICAL OPTICS | - |
dc.citation.volume | 19 | - |
dc.contributor.affiliatedAuthor | Chang, M | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.contributor.affiliatedAuthor | LEE, JONG BONG | - |
dc.identifier.scopusid | 2-s2.0-84891528219 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 7 | - |
dc.description.scptc | 7 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE VISCOSITY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | DIFFUSION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | MOBILITY | - |
dc.subject.keywordPlus | LIGANDS | - |
dc.subject.keywordAuthor | aptamer | - |
dc.subject.keywordAuthor | total internal reflection fluorescence microscopy | - |
dc.subject.keywordAuthor | single-molecule imaging | - |
dc.subject.keywordAuthor | insulin receptor | - |
dc.subject.keywordAuthor | diffusion | - |
dc.subject.keywordAuthor | cholesterol | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
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