Aptamer-based single-molecule imaging of insulin receptors in living cells
SCIE
SCOPUS
- Title
- Aptamer-based single-molecule imaging of insulin receptors in living cells
- Authors
- Chang, M; Kwon, M; Kim, S; Yunn, NO; Kim, D; Ryu, SH; LEE, JONG BONG
- Date Issued
- 2014-05
- Publisher
- SPIE
- Abstract
- We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-beta-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane. (C) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/14842
- DOI
- 10.1117/1.JBO.19.5.051204
- ISSN
- 1083-3668
- Article Type
- Article
- Citation
- JOURNAL OF BIOMEDICAL OPTICS, vol. 19, no. 5, 2014-05
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