Improvement of Hydrogen Production Yield by Rebalancing NADPH/NADH Ratio in a Recombinant Escherichia coil
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- Title
- Improvement of Hydrogen Production Yield by Rebalancing NADPH/NADH Ratio in a Recombinant Escherichia coil
- Authors
- Jong Moon Park; Kim, YM; Min, BE; Jung, GY; Park, JM
- Date Issued
- 2011-08
- Publisher
- American Scientific Publishers
- Abstract
- The NAD(P)H- or ferredoxin-dependent hydrogen production pathway is known to have a huge potential for improving its production yield compared to the formate hydrogen lyase (FHL) route. To improve the hydrogen production yield through this pathway, rebalancing the NADPH/NADH ratio based on the cofactor preference is important, in addition to enhancing the NAD(P)H pool by reducing the competing pathways. NADPH:ferredoxin oxidoreductase coupled with [FeFe]-hydrogenase preferably utilizes NADPH as an electron donor. Therefore, directing NADPH should be required to improve the hydrogen production yield. For balancing the NADPH/NADH ratio, nicotinamide nucleotide transhydrogenase has a key role, and Escherichia coli has the two transhydrogenases, PntAB and SthA. In this study, several recombinants were constructed to up-regulate pntAB or to down-regulate sthA in the competing-/dhA-deleted E. coli expressing [FeFe]-hydrogenase system. As a result, an about 3.9-fold increased hydrogen yield was observed in the pntAB-overexpressed and sthA-deleted strain. Therefore, the combination of IdhA down-regulation and NADPH/NADH ratio rebalancing via transhydrogenase engineering can improve the hydrogen production yield in the NADPH- or ferredoxin-dependent hydrogen production pathway.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/15593
- DOI
- 10.1166/JNO.2011.1182
- ISSN
- 1555-130X
- Article Type
- Article
- Citation
- JOURNAL OF NANOELECTRONICS AND OPTOELECTRONICS, vol. 6, no. 3, page. 343 - 347, 2011-08
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