Delivery of IL-12p40 ameliorates DSS-induced colitis by suppressing IL-17A expression and inflammation in the intestinal mucosa
SCIE
SCOPUS
- Title
- Delivery of IL-12p40 ameliorates DSS-induced colitis by suppressing IL-17A expression and inflammation in the intestinal mucosa
- Authors
- Doo-Jin Kim; Kwang-Soon Kim; Mi-Young Song; Sang- Hwan Seo; Su-Jin Kim; Bo-Gie Yang; Myoung-Ho Jang; Sung, YC
- Date Issued
- 2012-09
- Publisher
- Elsevier INC
- Abstract
- IL-12p40 homodimer is a natural antagonist of IL-12 and IL-23, which are potent pro-inflammatory cytokines required for Th1 and Th17 immune responses, respectively. It has been reported that Th17 response is involved in inflammatory bowel disease (IBD), a chronic disorder of the digestive system with steadily increasing incidence. Here, we investigated the effects of IL-12p40 delivered via recombinant adenovirus (rAd/IL-12p40) or mesenchymal stem cells (MSC/IL-12p40) in a dextran sulfate sodium salt (DSS)-induced colitis model. Injection of rAd/IL-12p40 or MSC/IL-12p40 efficiently attenuated colitis symptoms and tissue damage, Leading to an increased survival rate. Moreover, IL-12p40 delivery suppressed IL-17A, but enhanced IFN-gamma production from mesenteric lymph node cells, supporting the preferential suppression of IL-23 by IL-12p40 homodimer in vitro and the suppression of Th17 responses in vivo. Our results demonstrate that IL-12p40 delivery ameliorates DSS-induced colitis by suppressing IL-17A production and inflammation in the intestinal mucosa, providing an effective new therapeutic strategy for IBDs. (c) 2012 Elsevier Inc. All rights reserved.
- Keywords
- IL-12p40; DSS-induced colitis; Inflammatory bowel disease (IBD); IL-17A; MESENCHYMAL STEM-CELLS; SODIUM-INDUCED COLITIS; SEVERE CROHNS-DISEASE; TNBS-INDUCED COLITIS; CD4(+) T-CELLS; BOWEL-DISEASE; P40 HOMODIMER; STIMULATORY FACTOR; INTERFERON-GAMMA; MICE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/16366
- DOI
- 10.1016/J.CLIM.2012.06.009
- ISSN
- 1521-6616
- Article Type
- Article
- Citation
- CLINICAL IMMUNOLOGY, vol. 144, no. 3, page. 190 - 199, 2012-09
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.