The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.)
SCIE
SCOPUS
- Title
- The Vr-PLC3 gene encodes a putative plasma membrane-localized phosphoinositide-specific phospholipase C whose expression is induced by abiotic stress in mung bean (Vigna radiata L.)
- Authors
- Kim, YJ; Kim, JE; Lee, JH; Lee, MH; Jung, HW; Bahk, YY; Hwang, BK; Hwang, I; Kim, WT
- Date Issued
- 2004-01-02
- Publisher
- ELSEVIER SCIENCE BV
- Abstract
- Phosphoinositide-specific phospholipase C (PI-PLC) catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate inositol 1,4,5-trisphosphate and diacylglycerol, both of which act as secondary messengers in animal cells. In this report, we identified in Vigna radiata L. (mung bean) three distinct partial cDNAs (pVr-PLC1, pVr-PLC2, and pVrPLC3), which encode forms of putative PI-PLC. All three Vr-PLC genes were transcriptionally active and displayed unique patterns of expression. The Vr-PLC1 and Vr-PLC2 transcripts were constitutively expressed to varying degrees in every tissue of mung bean plants examined. In contrast, the Vr-PLC3 mRNA level was very low under normal growth conditions and was rapidly induced in an abscisic acid-independent manner under environmental stress conditions (drought and high salinity). An isolated genomic clone, about 8.2 kb in length, showed that Vr-PLC1 and Vr-PLC3 are in tandem array in the mung bean genome. The predicted primary sequence of Vr-PLC3 (M-r = 67.4 kDa) is reminiscent of the delta-isoform of animal enzymes which contain core sequences found in typical PI-PLCs, such as the catalytic domain comprising X and Y motifs, a lipid-binding C2 domain, and the less conserved EF-hand domain. Results of in vivo targeting experiment using a green fluorescent protein (GFP) showed that the GFP-Vr-PLC3 fusion protein was localized primarily to the plasma membrane of the Arabidopsis protoplast. The C2 domain was essential for Vr-PLC3 to be targeted to the plasma membrane. The possible biological functions of stress-responsive Vr-PLC3 in mung bean plants are discussed. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- Keywords
- abiotic stress; calcium; differential gene expression; inositol 1,4,5-trisphosphate; phospholipase C; plasma membrane; Vigna radiata; LENGTH CDNA MICROARRAY; OXIDASE MESSENGER-RNA; ARABIDOPSIS-THALIANA; SIGNAL-TRANSDUCTION; ABSCISIC-ACID; PLANT-CELLS; HYPEROSMOTIC STRESS; DROUGHT; ISOFORMS; CALCIUM
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18141
- DOI
- 10.1016/S0014-5793(03)01388-7
- ISSN
- 0014-5793
- Article Type
- Article
- Citation
- FEBS LETTERS, vol. 556, no. 1-3, page. 127 - 136, 2004-01-02
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.