DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, IH | - |
dc.contributor.author | Lee, S | - |
dc.contributor.author | Park, JB | - |
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Lee, CS | - |
dc.contributor.author | Hur, EM | - |
dc.contributor.author | Kim, IS | - |
dc.contributor.author | Kim, KT | - |
dc.contributor.author | Yagisawa, H | - |
dc.contributor.author | Suh, PG | - |
dc.contributor.author | Ryu, SH | - |
dc.date.accessioned | 2016-03-31T12:50:55Z | - |
dc.date.available | 2016-03-31T12:50:55Z | - |
dc.date.created | 2009-02-28 | - |
dc.date.issued | 2003-05-16 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.other | 2003-OAK-0000003390 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/18539 | - |
dc.description.abstract | The epidermal growth factor (EGF) receptor has an important role in cellular proliferation, and the enzymatic activity of phospholipase C (PLC)-gamma1 is regarded to be critical for EGF-induced mitogenesis. In this study, we report for the first time a phospholipase complex composed of PLC-gamma1 and phospholipase D2 (PLD2). PLC-gamma1 is co-immunoprecipitated with PLD2 in COS-7 cells. The results of in vitro binding analysis and co-immunoprecipitation analysis in COS-7 cells show that the Src homology (SH) 3 domain of PLC-gamma1 binds to the proline-rich motif within the Phox homology (PX) domain of PLD2. The interaction between PLC-gamma1 and PLD2 is EGF stimulation-dependent and potentiates EGF-induced inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ increase. Mutating Pro-145 and Pro-148 within the PX domain of PLD2 to leucines disrupts the interaction between PLC-gamma1 and PLD2 and fails to potentiate EGF-induced IP3 formation and Ca2+ increase. However, neither PLD2 wild type nor PLD2 mutant affects the EGF-induced tyrosine phosphorylation of PLC-gamma1. These findings suggest that, upon EGF stimulation, PLC-gamma1 directly interacts with PLD2 and this interaction is important for PLC-gamma1 activity. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLO | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.title | The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1074/jbc.M208438200 | - |
dc.author.google | Jang, IH | - |
dc.author.google | Lee, S | - |
dc.author.google | Park, JB | - |
dc.author.google | Kim, JH | - |
dc.author.google | Lee, CS | - |
dc.author.google | Hur, EM | - |
dc.author.google | Kim, IS | - |
dc.author.google | Kim, KT | - |
dc.author.google | Yagisawa, H | - |
dc.author.google | Suh, PG | - |
dc.author.google | Ryu, SH | - |
dc.relation.volume | 278 | - |
dc.relation.issue | 20 | - |
dc.relation.startpage | 18184 | - |
dc.relation.lastpage | 18190 | - |
dc.contributor.id | 10104775 | - |
dc.relation.journal | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.relation.sci | SCI | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.278, no.20, pp.18184 - 18190 | - |
dc.identifier.wosid | 000182838300078 | - |
dc.date.tcdate | 2019-01-01 | - |
dc.citation.endPage | 18190 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 18184 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 278 | - |
dc.contributor.affiliatedAuthor | Kim, KT | - |
dc.contributor.affiliatedAuthor | Suh, PG | - |
dc.contributor.affiliatedAuthor | Ryu, SH | - |
dc.identifier.scopusid | 2-s2.0-0038381419 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 40 | - |
dc.description.isOpenAccess | N | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | PROTEIN-KINASE-C | - |
dc.subject.keywordPlus | RECEPTOR TYROSINE KINASE | - |
dc.subject.keywordPlus | SRC HOMOLOGY DOMAINS | - |
dc.subject.keywordPlus | PHOSPHATIDIC-ACID | - |
dc.subject.keywordPlus | SH3 DOMAIN | - |
dc.subject.keywordPlus | MEMBRANE DOMAINS | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE | - |
dc.subject.keywordPlus | EGF RECEPTOR | - |
dc.subject.keywordPlus | PX DOMAINS | - |
dc.subject.keywordPlus | D ISOZYMES | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
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