The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling
SCIE
SCOPUS
- Title
- The direct interaction of phospholipase C-gamma 1 with phospholipase D2 is important for epidermal growth factor signaling
- Authors
- Jang, IH; Lee, S; Park, JB; Kim, JH; Lee, CS; Hur, EM; Kim, IS; Kim, KT; Yagisawa, H; Suh, PG; Ryu, SH
- Date Issued
- 2003-05-16
- Publisher
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLO
- Abstract
- The epidermal growth factor (EGF) receptor has an important role in cellular proliferation, and the enzymatic activity of phospholipase C (PLC)-gamma1 is regarded to be critical for EGF-induced mitogenesis. In this study, we report for the first time a phospholipase complex composed of PLC-gamma1 and phospholipase D2 (PLD2). PLC-gamma1 is co-immunoprecipitated with PLD2 in COS-7 cells. The results of in vitro binding analysis and co-immunoprecipitation analysis in COS-7 cells show that the Src homology (SH) 3 domain of PLC-gamma1 binds to the proline-rich motif within the Phox homology (PX) domain of PLD2. The interaction between PLC-gamma1 and PLD2 is EGF stimulation-dependent and potentiates EGF-induced inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ increase. Mutating Pro-145 and Pro-148 within the PX domain of PLD2 to leucines disrupts the interaction between PLC-gamma1 and PLD2 and fails to potentiate EGF-induced IP3 formation and Ca2+ increase. However, neither PLD2 wild type nor PLD2 mutant affects the EGF-induced tyrosine phosphorylation of PLC-gamma1. These findings suggest that, upon EGF stimulation, PLC-gamma1 directly interacts with PLD2 and this interaction is important for PLC-gamma1 activity.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/18539
- DOI
- 10.1074/jbc.M208438200
- ISSN
- 0021-9258
- Article Type
- Article
- Citation
- JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 20, page. 18184 - 18190, 2003-05-16
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