Characteristics of DNA microarrays fabricated on various aminosilane layers
SCIE
SCOPUS
- Title
- Characteristics of DNA microarrays fabricated on various aminosilane layers
- Authors
- Oh, SJ; Cho, SJ; Kim, CO; Park, JW
- Date Issued
- 2002-03-05
- Publisher
- AMER CHEMICAL SOC
- Abstract
- Four kinds of aminosilane layers on glass slides or silicon wafers were prepared. The amine densities of the layers prepared with aminopropylmonoethoxydimethylsilane (APDES), aminopropylmonoethoxydimethylsilane (APMES), a mixture of (aminopropyl)triethoxysilane (APTES) and n-butyltrimethoxysilane (n-BTMS) (v/v = 1:10) are 4.0(+/-0.8), 1.0(+/-0.2), and 0.30(+/-0.6) amine/nm(2), respectively. A substrate with much higher amine density, that is, 40(+/-8) amines/nm(2) was also prepared by allowing aziridine to polymerize on the APDES-treated substrate. AFM revealed that APDES-, APMES-, and APTES/n-BTMS-treated surfaces were relatively flat; on the other hand, an aziridine-treated surface showed embossed morphology. The amine substrates were allowed to react with a heterobifunctional linker succinimidyl 4-maleimido butyrate (SMB), and subsequently pentadecadeoxynucleotides were microarrayed on the SMB-treated substrates. Characteristics of the DNA microarrays including the dynamic range, the mismatch discrimination efficiency, and so forth were examined. Noteworthily, DNA microarrays on the aziridine-polymerized substrate showed much higher fluorescence intensity. At the same time, DNA microarrays from these four substrates were able to discriminate internal- and terminal-mismatched pairs, but the fluorescence ratio was far from the one that thermodynamics implies.
- Keywords
- DOUBLE-STRANDED-DNA; SELF-ASSEMBLED MONOLAYERS; ATOMIC-FORCE MICROSCOPY; COVALENT IMMOBILIZATION; DSDNA MICROARRAYS; SURFACE-DENSITY; GOLD ELECTRODES; GLASSY-CARBON; THIN-LAYERS; AMINE GROUP
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19187
- DOI
- 10.1021/LA0113522
- ISSN
- 0743-7463
- Article Type
- Article
- Citation
- LANGMUIR, vol. 18, no. 5, page. 1764 - 1769, 2002-03-05
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- There are no files associated with this item.
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