The enhanced effect of a hexameric deoxyriboguanosine run conjugation to CpG oligodeoxynucleotides on protection against allergic asthma
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- Title
- The enhanced effect of a hexameric deoxyriboguanosine run conjugation to CpG oligodeoxynucleotides on protection against allergic asthma
- Authors
- Park, Y; Chang, YS; Lee, SW; Cho, SY; Kim, YK; Min, KU; Kim, YY; Cho, SH; Sung, YC
- Date Issued
- 2001-10
- Publisher
- MOSBY, INC
- Abstract
- Background: Oligodeoxynucleotides containing a CpG motif (CpG ODNs), as potent inducers of T(H)1 immunity, are considered promising candidates for immune modulation in asthma. We have previously demonstrated that conjugation of a hexameric deoxyriboguanosine run to the 3' terminus (3' dG(6)-run) of phosphodiester (PE) CpG ODNs enhanced their immunostimulatory activities in vitro. Objective: This study aimed to evaluate the effect of a 3' dG(6)-run conjugation to PE or phosphorothioate (PS) CpG ODNs on protection against murine allergic asthma in vivo. Methods: Balb/c mice were sensitized to ovalbumin by intraperitoneal injection with or without CpG ODNs (PS CpG ODNs, PE CpG ODNs, and those with 3'dG(6)-run) and subsequently challenged with ovalbumin. We evaluated airway hyperresponsiveness, eosinophil proportion in bronchoalveolar lavage fluid, airway inflammation, and ovalbumin-specific antibody responses. Results: The conjugation of a 3' dG(6)-run to PE CpG ODNs enhanced the production of IFN-gamma from ovalbumin-specific T-H cells and prevented the development of asthma in terms of airway hyperresponsiveness, airway eosinophilia, and ovalbumin-specific IgE responses; these effects were comparable to those of PS CpG ODNs. Enhanced effects of the 3' dG(6)-run were also observed in PS CpG ODNs, though they were lower than those in PE CpG ODNs. Conclusion: This study suggests that conjugation of a 3' dG(6)-run to CpG ODNs might provide an effective method for immune modulation of allergic asthma.
- Keywords
- asthma; CpG oligodeoxynucleotides; 3 ' dG(6)-run; phosphodiester; T(H)1; immune modulation; IMMUNOSTIMULATORY DNA-SEQUENCES; CD8 T-CELLS; BACTERIAL-DNA; PHOSPHOROTHIOATE OLIGODEOXYNUCLEOTIDES; AIRWAY HYPERRESPONSIVENESS; INFLAMMATION; MOTIFS; MURINE; MICE; EOSINOPHILIA
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/19338
- DOI
- 10.1067/mai.2001.118517
- ISSN
- 0091-6749
- Article Type
- Article
- Citation
- JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, vol. 108, no. 4, page. 570 - 576, 2001-10
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