Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen
SCIE
SCOPUS
- Title
- Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen
- Authors
- Kim, HY; Ahn, BY; Cho, Y
- Date Issued
- 2001-01-15
- Publisher
- OXFORD UNIV PRESS
- Abstract
- Inactivation of the retinoblastoma (Rb) tumor suppressor by Simian virus 40 (SV40) large T antigen is one of the central features of tumorigenesis induced by SV40. Both the N-terminal J domain and the LxCxE motif of large T antigen are required for inactivation of Rb, The crystal structure of the N-terminal region (residues 7-117) of SV40 large T antigen bound to the pocket domain of Rb reveals that large T antigen contains a four-helix bundle, and residues from helices alpha2 and alpha4 and from a loop containing the LxCxE motif participate in the interactions with Rb. The two central helices and a connecting loop in large T antigen have structural similarities with the J domains of the molecular chaperones DnaJ and HDJ-1, suggesting that large T antigen may use a chaperone mechanism for its biological function. However, there are significant differences between large T antigen and the molecular chaperones in other regions and these differences are likely to provide the specificity needed for large T antigen to inactivate Rb.
- Keywords
- chaperone mechanism; Rb tumor suppressor; SV40 large T antigen; viral oncogene; SIMIAN-VIRUS-40 LARGE-T; ESCHERICHIA-COLI DNAJ; CELL-CYCLE CONTROL; J-DOMAIN; GENE-PRODUCT; TRANSCRIPTION FACTOR; SUSCEPTIBILITY GENE; MOLECULAR CHAPERONE; CRYSTAL-STRUCTURE; FAMILY PROTEINS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/20998
- DOI
- 10.1093/emboj/20.1.295
- ISSN
- 0261-4189
- Article Type
- Article
- Citation
- EMBO JOURNAL, vol. 20, no. 1-2, page. 295 - 304, 2001-01-15
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.