The synthetic chemoattractant peptide WKYMVm induces superoxide production by human eosinophils via the phosphoinositide 3-kinase-mediated activation of ERK1/2
SCIE
SCOPUS
- Title
- The synthetic chemoattractant peptide WKYMVm induces superoxide production by human eosinophils via the phosphoinositide 3-kinase-mediated activation of ERK1/2
- Authors
- Shin, MH; Lee, YA; Bae, YS; Kita, H; Kim, K; Ryu, SH
- Date Issued
- 2005-01
- Publisher
- KARGER
- Abstract
- Background: Eosinophils play a key role in allergic inflammation and parasitic infections. The synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), has been previously shown to activate eosinophils and thus to enhance respiratory burst through the formyl peptide receptors. Objective: This study was undertaken to determine the intracellular signaling pathway involved in WKYMVm-stimulated superoxide production by human eosinophils. Methods: Purified eosinophils from peripheral blood were stimulated with various concentrations (10(-3) to 10 mu M) of WKYMVm and the involvement of PI3-kinase and MAP kinases in WKYMVm-triggered superoxide production was investigated using pharmacological inhibitors. Results: WKYMVm-induced superoxide production by eosinophils was strongly inhibited by pretreatment with the PI3-kinase inhibitor LY294002. In addition, pretreatment with the ERK1/2 kinase inhibitor PD98059 resulted in marked inhibition of superoxide production induced by WKYMVm. Indeed, WKYMVm strongly induced phosphorylation of ERK1/2. The ERK1/2 activation by the peptide was transient and peaked after 2 min of stimulation. Furthermore, ERK1/2 activation by WKYMVm was completely inhibited by pretreatment with the PI3-kinase inhibitor LY294002, but not by the PKC inhibitor Ro-31-8220. Conclusion: These results suggest that WKYMVm stimulates human eosinophils to induce superoxide production via a PI3-kinase-mediated ERK1/2 pathway. Copyright (C) 2005 S. Karger AG, Basel.
- Keywords
- eosinophils; WKYMVm; superoxide; phosphoinositide 3-kinase; ERK1/2; VAL-D-MET; HUMAN NEUTROPHILS; HUMAN MONOCYTES; RECEPTOR; P47(PHOX); PHOSPHORYLATION; GENERATION; KINASE; AKT; HYDROLYSIS
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/24565
- DOI
- 10.1159/000085428
- ISSN
- 1018-2438
- Article Type
- Article
- Citation
- INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, vol. 137, page. 21 - 26, 2005-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.