A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction
SCIE
SCOPUS
- Title
- A functional carbohydrate chip platform for analysis of carbohydrate-protein interaction
- Authors
- Seo, JH; Kim, CS; Hwang, BH; Cha, HJ
- Date Issued
- 2010-05-28
- Publisher
- IOP PUBLISHING LTD
- Abstract
- A carbohydrate chip based on glass or other transparent surfaces has been suggested as a potential tool for high-throughput analysis of carbohydrate-protein interactions. Here we proposed a facile, efficient, and cost-effective method whereby diverse carbohydrate types are modified in a single step and directly immobilized onto a glass surface, with retention of functional orientation. We modified various types of carbohydrates by reductive amination, in which reducing sugar groups were coupled with 4-(2-aminoethyl)aniline, which has di-amine groups at both ends. The modified carbohydrates were covalently attached to an amino-reactive NHS-activated glass surface by formation of stable amide bonds. This proposed method was applied for efficient construction of a carbohydrate microarray to analyze carbohydrate-protein interactions. The carbohydrate chip prepared using our method can be successfully used in diverse biomimetic studies of carbohydrates, including carbohydrate-biomolecule interactions, and carbohydrate sensor chip or microarray development for diagnosis and screening.
- Keywords
- SELF-ASSEMBLED MONOLAYERS; CHOLERA-TOXIN; SUGAR CHAINS; OLIGOSACCHARIDE MICROARRAYS; BINDING; SURFACE; IMMOBILIZATION; GLYCOSYLATION; SPECIFICITY; RECEPTOR
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25354
- DOI
- 10.1088/0957-4484/21/21/215101
- ISSN
- 0957-4484
- Article Type
- Article
- Citation
- NANOTECHNOLOGY, vol. 21, no. 21, 2010-05-28
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- There are no files associated with this item.
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