Promotion of osteoblast proliferation on complex coacervation-based hyaluronic acid - recombinant mussel adhesive protein coatings on titanium
SCIE
SCOPUS
- Title
- Promotion of osteoblast proliferation on complex coacervation-based hyaluronic acid - recombinant mussel adhesive protein coatings on titanium
- Authors
- Hwang, DS; J. Herbert Waite; Matthew Tirrell
- Date Issued
- 2010-02
- Publisher
- Elsevier B.V
- Abstract
- Many biological polyelectrolytes are capable of undergoing a fluid-fluid phase separation known as complex coacervation. Coacervates were prepared using hyaluronic acid (HA) and a recombinant fusion protein consisting of mussel adhesive motifs and the RGD peptide (fp-151-RGD). The low interfacial energy of the coacervate was exploited to coat titanium (Ti), a metal widely used in implant materials. The coacervate effectively distributed both HA and fp-151-RGD over the Ti surfaces and enhanced osteoblast proliferation. Approximately half of total fp-151-RGD and HA in the solution transferred to the titanium surface within 2 h. Titanium coated with coacervates having high residual negative surface charge showed the highest cell proliferation of preosteoblast cells (MC-3T3) compared to the treatments tested. Indeed, MC-3T3 cells on complex coacervate coated titanium foils exhibited over 5 times greater cell proliferation than bare, HA coated or fp-151-RGD coated titanium. (C) 2009 Elsevier Ltd. All rights reserved.
- Keywords
- Complex coacervate; Hyaluronic acid; Mussel adhesive protein; fp-151-RGD; MC-3T3; Biocoating; CELL-ADHESION; POLYELECTROLYTE MULTILAYERS; RGD; GROWTH; CALIFORNICA; CULTURE
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/25712
- DOI
- 10.1016/J.BIOMATERIALS.2009.10.041
- ISSN
- 0142-9612
- Article Type
- Article
- Citation
- BIOMATERIALS, vol. 31, no. 6, page. 1080 - 1084, 2010-02
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