Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator
SCIE
SCOPUS
- Title
- Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator
- Authors
- Cheow, LF; Ko, SH; Kim, SJ; Kang, KH; Han, J
- Date Issued
- 2010-04-15
- Publisher
- AMER CHEMICAL SOC
- Abstract
- We developed a novel method to increase the sensitivity of standard enzyme-linked immunosorbent assay (ELISA) using a multiplexed electrokinetic concentration chip. The poly(dimethylsiloxane) (PDMS) molecular concentrator(1) was used to trap and collect charged fluorescent product of target-bound enzyme turnover reaction of ELISA that occurred in a standard 96 well plate. Detection sensitivities of both prostate specific antigen (PSA) and CA 19-9 (a human pancreatic and gastrointestinal cancer marker) ELISAs in serum are enhanced similar to 100 fold with a low CV of <17%. We also integrated this method with an on-chip bead-based ELISA that lends itself toward a fully automated on-chip diagnostic device. Detection sensitivity of microfluidic bead-based CA 19-9 ELISA in serum is enhanced similar to 65 fold compared to the results without the electrokinetic accumulation step. This chip can be directly applied to enhance the readout sensitivity of a wide range of existing ELISA kits at concentrations below the current detection limit.
- Keywords
- PROSTATE-SPECIFIC ANTIGEN; PRECONCENTRATION; PROTEINS; SYSTEM; CANCER
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/26071
- DOI
- 10.1021/AC9024335
- ISSN
- 0003-2700
- Article Type
- Article
- Citation
- ANALYTICAL CHEMISTRY, vol. 82, no. 8, page. 3383 - 3388, 2010-04-15
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- There are no files associated with this item.
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