High-resolution genome-wide mapping of histone modifications
SCIE
SCOPUS
- Title
- High-resolution genome-wide mapping of histone modifications
- Authors
- Roh, TY; Ngau, WC; Cui, KR; L; sman, D; Zhao, KJ
- Date Issued
- 2004-08
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- The expression patterns of eukaryotic genomes are controlled by their chromatin structure, consisting of nucleosome subunits in which DNA of approximately 146 bp is wrapped around a core of 8 histone molecules(1). Post-translational histone modifications play an essential role in modifying chromatin structure(.)(2) Here we apply a combination of SAGE 3 and chromatin immunoprecipitation (ChIP) protocols to determine the distribution of hyperacetylated histones H3 and H4 in the Saccharomyces cerevisiae genome. We call this approach genome-wide mapping technique (GMAT). Using GMAT, we find that the highest acetylation levels are detected in the 5 end of a gene's coding region, but not in the promoter. Furthermore, we show that the histone acetyltransferase, GCN5p, regulates H3 acetylation in the promoter and 5 end of the coding regions. These findings indicate that GMAT should find valuable applications in mapping target sites of chromatin-modifying enzymes.
- Keywords
- YEAST HETEROCHROMATIN; ACETYLATION; H4; TRANSCRIPTOME; DEACETYLATION; NUCLEOSOME; TELOMERES; PROMOTER; GCN5P
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/28975
- DOI
- 10.1038/NBT990
- ISSN
- 1087-0156
- Article Type
- Article
- Citation
- NATURE BIOTECHNOLOGY, vol. 22, no. 8, page. 1013 - 1016, 2004-08
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