Active Caspase-1-Mediated Secretion of Retinoic Acid Inducible Gene-I
SCIE
SCOPUS
- Title
- Active Caspase-1-Mediated Secretion of Retinoic Acid Inducible Gene-I
- Authors
- Kim, MJ; Yoo, JY
- Date Issued
- 2008-11-15
- Publisher
- AMER ASSOC IMMUNOLOGISTS
- Abstract
- Caspase-1 is an inflammatory caspase that controls the activation and secretion of the inflammatory cytokines, IL-1 beta and IL-18. We observed that cellular levels of retinoic acid-inducible gene-I (RIG-I) were enhanced when the pan-caspase inhibitor Z-VAD-fmk or caspase-1-specific inhibitor Z-WEHD-fmk blocked caspase activity. Overexpression of caspase-1 reduced cellular levels of RIG-I and inhibited RIG-I-mediated signaling activity. Enzymatic activity of caspase-1 was necessary to control RIG-I, although it was not a substrate of proteolytic cleavage by caspase-1. Caspase-1 physically interacted with full length RIG-I, but not with mutant forms lacking either the amino- or carboxyl-terminal domains. RIG-I was present in the supernatant of cells transfected with active caspase-1 but not with caspase-4. Stimulating cells with LPS and ATP also induced secretion of endogenous RIG-I in macrophages. Our data suggest a novel mechanism that negatively regulates RIG-I-mediated signaling activity via caspase-1-dependent secretion of RIG-I protein. The Journal of Immunology, 2008, 181: 7324-7331.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29359
- DOI
- 10.4049/jimmunol.181.10.7324
- ISSN
- 0022-1767
- Article Type
- Article
- Citation
- JOURNAL OF IMMUNOLOGY, vol. 181, no. 10, page. 7324 - 7331, 2008-11-15
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