DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, SY | - |
dc.contributor.author | Bae, WJ | - |
dc.contributor.author | Kim, JJ | - |
dc.contributor.author | Yeom, KH | - |
dc.contributor.author | Kim, VN | - |
dc.contributor.author | Cho, Y | - |
dc.date.accessioned | 2016-04-01T09:08:11Z | - |
dc.date.available | 2016-04-01T09:08:11Z | - |
dc.date.created | 2009-03-05 | - |
dc.date.issued | 2007-09 | - |
dc.identifier.issn | 1545-9985 | - |
dc.identifier.other | 2007-OAK-0000010773 | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/29519 | - |
dc.description.abstract | A complex of Drosha with DGCR8 ( or its homolog Pasha) cleaves primary microRNA ( pri- miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri- miRNAs. To clarify the molecular basis underlying recognition of pri- miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core ( DGCR8S, residues 493 - 720). In the structure, the two double- stranded RNA - binding domains ( dsRBDs) are arranged with pseudo two- fold symmetry and are tightly packed against the C- terminal helix. The H2 helix in each dsRBD is important for recognition of pri- miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri- miRNA in two possible orientations. We propose a model for DGCR8' s recognition of pri- miRNA. | - |
dc.description.statementofresponsibility | X | - |
dc.language | English | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.relation.isPartOf | NATURE STRUCTURAL & MOLECULAR BIOLOGY | - |
dc.subject | RNA-BINDING DOMAIN | - |
dc.subject | DIGEORGE-SYNDROME | - |
dc.subject | MOLECULAR-BASIS | - |
dc.subject | NUCLEAR EXPORT | - |
dc.subject | MICROPROCESSOR COMPLEX | - |
dc.subject | DROSHA-DGCR8 COMPLEX | - |
dc.subject | PRIMARY MICRORNAS | - |
dc.subject | ENZYME COMPLEX | - |
dc.subject | C-ELEGANS | - |
dc.subject | PROTEIN | - |
dc.title | Crystal structure of human DGCR8 core | - |
dc.type | Article | - |
dc.contributor.college | 생명과학과 | - |
dc.identifier.doi | 10.1038/NSMB1294 | - |
dc.author.google | Sohn, SY | - |
dc.author.google | Bae, WJ | - |
dc.author.google | Kim, JJ | - |
dc.author.google | Yeom, KH | - |
dc.author.google | Kim, VN | - |
dc.author.google | Cho, Y | - |
dc.relation.volume | 14 | - |
dc.relation.issue | 9 | - |
dc.relation.startpage | 847 | - |
dc.relation.lastpage | 853 | - |
dc.contributor.id | 10082321 | - |
dc.relation.journal | NATURE STRUCTURAL & MOLECULAR BIOLOGY | - |
dc.relation.index | SCI급, SCOPUS 등재논문 | - |
dc.collections.name | Journal Papers | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | NATURE STRUCTURAL & MOLECULAR BIOLOGY, v.14, no.9, pp.847 - 853 | - |
dc.identifier.wosid | 000249276500012 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 853 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 847 | - |
dc.citation.title | NATURE STRUCTURAL & MOLECULAR BIOLOGY | - |
dc.citation.volume | 14 | - |
dc.contributor.affiliatedAuthor | Cho, Y | - |
dc.identifier.scopusid | 2-s2.0-34548480185 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 77 | - |
dc.description.scptc | 81 | * |
dc.date.scptcdate | 2018-05-121 | * |
dc.type.docType | Article | - |
dc.subject.keywordPlus | RNA-BINDING DOMAIN | - |
dc.subject.keywordPlus | DIGEORGE-SYNDROME | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | NUCLEAR EXPORT | - |
dc.subject.keywordPlus | MICROPROCESSOR COMPLEX | - |
dc.subject.keywordPlus | DROSHA-DGCR8 COMPLEX | - |
dc.subject.keywordPlus | PRIMARY MICRORNAS | - |
dc.subject.keywordPlus | ENZYME COMPLEX | - |
dc.subject.keywordPlus | C-ELEGANS | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Cell Biology | - |
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