Crystal structure of human DGCR8 core
SCIE
SCOPUS
- Title
- Crystal structure of human DGCR8 core
- Authors
- Sohn, SY; Bae, WJ; Kim, JJ; Yeom, KH; Kim, VN; Cho, Y
- Date Issued
- 2007-09
- Publisher
- NATURE PUBLISHING GROUP
- Abstract
- A complex of Drosha with DGCR8 ( or its homolog Pasha) cleaves primary microRNA ( pri- miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri- miRNAs. To clarify the molecular basis underlying recognition of pri- miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core ( DGCR8S, residues 493 - 720). In the structure, the two double- stranded RNA - binding domains ( dsRBDs) are arranged with pseudo two- fold symmetry and are tightly packed against the C- terminal helix. The H2 helix in each dsRBD is important for recognition of pri- miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri- miRNA in two possible orientations. We propose a model for DGCR8' s recognition of pri- miRNA.
- Keywords
- RNA-BINDING DOMAIN; DIGEORGE-SYNDROME; MOLECULAR-BASIS; NUCLEAR EXPORT; MICROPROCESSOR COMPLEX; DROSHA-DGCR8 COMPLEX; PRIMARY MICRORNAS; ENZYME COMPLEX; C-ELEGANS; PROTEIN
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/29519
- DOI
- 10.1038/NSMB1294
- ISSN
- 1545-9985
- Article Type
- Article
- Citation
- NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 14, no. 9, page. 847 - 853, 2007-09
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