Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System
SCIE
SCOPUS
- Title
- Isolation of High-Purity Extracellular Vesicles by Extracting Proteins Using Aqueous Two-Phase System
- Authors
- Kim, J; Shin, H; Kim, J; Kim, J; Park, J
- Date Issued
- 2015-06-19
- Publisher
- Public Library of Science
- Abstract
- We present a simple and rapid method to isolate extracellular vesicles (EVs) by using a polyethylene glycol/dextran aqueous two-phase system (ATPS). This system isolated more than similar to 75% of melanoma-derived EVs from a mixture of EVs and serum proteins. To increase the purity of EVs, a batch procedure was combined as additional steps to remove protein contaminants, and removed more than similar to 95% of the protein contaminants. We also performed RT-PCR and western blotting to verify the diagnostic applicability of the isolated EVs, and detected mRNA derived from melanoma cells and CD81 in isolated EVs.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/35603
- DOI
- 10.1371/JOURNAL.PONE.0129760
- ISSN
- 1932-6203
- Article Type
- Article
- Citation
- PLoS ONE, vol. 10, no. 6, 2015-06-19
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