DC Field | Value | Language |
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dc.contributor.author | YUN, EUN JIN | - |
dc.contributor.author | Lim, Kyu | - |
dc.contributor.author | Kang, Yong-Sun | - |
dc.contributor.author | Son, Mee-Young | - |
dc.contributor.author | Park, Chung | - |
dc.contributor.author | Song, Kyoung-sub | - |
dc.contributor.author | Kim, Jong-Seok | - |
dc.contributor.author | Kim, Young-Rae | - |
dc.contributor.author | Park, Jong-Il | - |
dc.contributor.author | Yoon, Wan-Hee | - |
dc.contributor.author | Park, Seung-Kiel | - |
dc.contributor.author | Hwang, Byung-Doo | - |
dc.date.accessioned | 2018-01-09T09:04:09Z | - |
dc.date.available | 2018-01-09T09:04:09Z | - |
dc.date.created | 2017-11-14 | - |
dc.date.issued | 2005-09 | - |
dc.identifier.issn | 1021-335X | - |
dc.identifier.uri | https://oasis.postech.ac.kr/handle/2014.oak/40849 | - |
dc.description.abstract | To gain insight on the role of transacting factors in the regulatory mechanism of H2B histone gene expression during the differentiation of HL-60 cells by 12-O-tetradecanoylphorbol 13-acetate (TPA), the binding pattern of nuclear proteins to various elements in the human H2B histone gene upstream region have been investigated with DNase 1 footprinting and DNA mobility shift assay. The level of H2B histone mRNA rapidly reduced at 24 h in TPA-treated HL-60 cells. The H2B histone mRNA was repressed in proportion to the concentration of TPA. In DNase 1 footprinting analysis, one nuclear factor (octamer-binding transcription factor, OTF) bound at -42 bp (octamer motif), before and after TPA-induced differentiation of HL-60 cells. One DNA-protein complex (OTF) was formed by DNA mobility shift assay when octamer element was incubated with nuclear extract of undifferentiated HL-60 cells. In DNA mobilith shift assay, OTF vanished, and phosphorylated OTF (p-OTF) newly appeared during TPA-induced differentiation. p-OTF was not detected after pretreatment of the protein kinase C inhibitor, staurosporin, but was not changed after CHX treatment. TPA-induced repression of H2B histone mRNA was also restored after pretreatment of staurosporin. These results suggest that OTF is phosphorylated by protein kinase C during TPA-induced differentiation of HL-60 and the transcriptional repression of the H2B histone gene may be | - |
dc.language | English | - |
dc.publisher | SPANDIDOS PUBL LTD | - |
dc.relation.isPartOf | ONCOLOGY REPORTS | - |
dc.subject | S-PHASE | - |
dc.subject | DIMETHYL-SULFOXIDE | - |
dc.subject | DNA REPLICATION | - |
dc.subject | LEUKEMIA-CELLS | - |
dc.subject | RETINOIC ACID | - |
dc.subject | CYCLE | - |
dc.subject | EXPRESSION | - |
dc.subject | PROMOTER | - |
dc.subject | PROTEIN | - |
dc.subject | PURIFICATION | - |
dc.title | Phosphorylation of octamer-binding transcriptional factor may be correlated with H2B histone gene repression during 12-O-tetradecanoylphorbol 13 acetate-dependent differentiation of HL-60 cells | - |
dc.type | Article | - |
dc.identifier.doi | 10.3892/or.14.3.727 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | ONCOLOGY REPORTS, v.14, no.3, pp.727 - 731 | - |
dc.identifier.wosid | 000231047600023 | - |
dc.date.tcdate | 2019-02-01 | - |
dc.citation.endPage | 731 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 727 | - |
dc.citation.title | ONCOLOGY REPORTS | - |
dc.citation.volume | 14 | - |
dc.contributor.affiliatedAuthor | YUN, EUN JIN | - |
dc.identifier.scopusid | 2-s2.0-24644500095 | - |
dc.description.journalClass | 1 | - |
dc.description.journalClass | 1 | - |
dc.description.wostc | 2 | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | S-PHASE | - |
dc.subject.keywordPlus | DIMETHYL-SULFOXIDE | - |
dc.subject.keywordPlus | DNA REPLICATION | - |
dc.subject.keywordPlus | LEUKEMIA-CELLS | - |
dc.subject.keywordPlus | RETINOIC ACID | - |
dc.subject.keywordPlus | CYCLE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROMOTER | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordAuthor | H2B histone | - |
dc.subject.keywordAuthor | octamer binding transcriptional factor | - |
dc.subject.keywordAuthor | TPA | - |
dc.subject.keywordAuthor | HL-60 cell | - |
dc.subject.keywordAuthor | differentiation | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
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