Phosphorylation of octamer-binding transcriptional factor may be correlated with H2B histone gene repression during 12-O-tetradecanoylphorbol 13 acetate-dependent differentiation of HL-60 cells
SCIE
SCOPUS
- Title
- Phosphorylation of octamer-binding transcriptional factor may be correlated with H2B histone gene repression during 12-O-tetradecanoylphorbol 13 acetate-dependent differentiation of HL-60 cells
- Authors
- YUN, EUN JIN; Lim, Kyu; Kang, Yong-Sun; Son, Mee-Young; Park, Chung; Song, Kyoung-sub; Kim, Jong-Seok; Kim, Young-Rae; Park, Jong-Il; Yoon, Wan-Hee; Park, Seung-Kiel; Hwang, Byung-Doo
- Date Issued
- 2005-09
- Publisher
- SPANDIDOS PUBL LTD
- Abstract
- To gain insight on the role of transacting factors in the regulatory mechanism of H2B histone gene expression during the differentiation of HL-60 cells by 12-O-tetradecanoylphorbol 13-acetate (TPA), the binding pattern of nuclear proteins to various elements in the human H2B histone gene upstream region have been investigated with DNase 1 footprinting and DNA mobility shift assay. The level of H2B histone mRNA rapidly reduced at 24 h in TPA-treated HL-60 cells. The H2B histone mRNA was repressed in proportion to the concentration of TPA. In DNase 1 footprinting analysis, one nuclear factor (octamer-binding transcription factor, OTF) bound at -42 bp (octamer motif), before and after TPA-induced differentiation of HL-60 cells. One DNA-protein complex (OTF) was formed by DNA mobility shift assay when octamer element was incubated with nuclear extract of undifferentiated HL-60 cells. In DNA mobilith shift assay, OTF vanished, and phosphorylated OTF (p-OTF) newly appeared during TPA-induced differentiation. p-OTF was not detected after pretreatment of the protein kinase C inhibitor, staurosporin, but was not changed after CHX treatment. TPA-induced repression of H2B histone mRNA was also restored after pretreatment of staurosporin. These results suggest that OTF is phosphorylated by protein kinase C during TPA-induced differentiation of HL-60 and the transcriptional repression of the H2B histone gene may be
- Keywords
- S-PHASE; DIMETHYL-SULFOXIDE; DNA REPLICATION; LEUKEMIA-CELLS; RETINOIC ACID; CYCLE; EXPRESSION; PROMOTER; PROTEIN; PURIFICATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/40849
- DOI
- 10.3892/or.14.3.727
- ISSN
- 1021-335X
- Article Type
- Article
- Citation
- ONCOLOGY REPORTS, vol. 14, no. 3, page. 727 - 731, 2005-09
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