Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon
SCIE
SCOPUS
- Title
- Facile conversion of ATP-binding RNA aptamer to quencher-free molecular aptamer beacon
- Authors
- PARK, YOOJIN; NIM-ANUSSORNKUL, DUANGRAT; VILAIVAN, TIRAYUT; MORII, TAKASHI; KIM, BYEANG HYEAN
- Date Issued
- 2018-01
- Publisher
- ELSEVIER SCIENCE LTD
- Abstract
- We have developed RNA-based quencher-free molecular aptamer beacons (RNA-based QF-MABs) for the detection of ATP, taking advantage of the conformational changes associated with ATP binding to the ATP-binding RNA aptamer. The RNA aptamer, with its well-defined structure, was readily converted to the fluorescence sensors by incorporating a fluorophore into the loop region of the hairpin structure. These RNA-based QF-MABs exhibited fluorescence signals in the presence of ATP relative to their low background signals in the absence of ATP. The fluorescence emission intensity increased upon formation of a RNA-based QF-MAB·ATP complex. © 2017 Elsevier Ltd
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/41008
- DOI
- 10.1016/j.bmcl.2017.12.008
- ISSN
- 0960-894X
- Article Type
- Article
- Citation
- BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 28, no. 2, page. 77 - 80, 2018-01
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