A Chemical Probe that Labels Human Pluripotent Stem Cells
SCIE
SCOPUS
- Title
- A Chemical Probe that Labels Human Pluripotent Stem Cells
- Authors
- Hirata, Nao; Nakagawa, Masato; Fujibayashi, Yuto; Yamauchi, Kaori; Murata, Asako; Minami, Itsunari; Tomioka, Maiko; Kondo, Takayuki; Kuo, Ting-Fang; Endo, Hiroshi; Inoue, Haruhisa; Sato, Shin-ichi; Ando, Shin; Kawazoe, Yoshinori; Aiba, Kazuhiro; Nagata, Koh; Kawase, Eihachiro; Chang, Young-Tae; Suemori, Hirofumi; Eto, Koji; Nakauchi, Hiromitsu; Yamanaka, Shinya; Nakatsuji, Norio; Ueda, Kazumitsu; Uesugi, Motonari
- Date Issued
- 2014-03
- Publisher
- CELL PRESS
- Abstract
- A small-molecule fluorescent probe specific for human pluripotent stem cells would serve as a useful tool for basic cell biology research and stem cell therapy. Screening of fluorescent chemical libraries with human induced pluripotent stem cells (iPSCs) and subsequent evaluation of hit molecules identified a fluorescent compound (Kyoto probe 1 [KP-1]) that selectively labels human pluripotent stem cells. Our analyses indicated that the selectivity results primarily from a distinct expression pattern of ABC transporters in human pluripotent stem cells and from the transporter selectivity of KP-1. Expression of ABCB1 (MDR1) and ABCG2 (BCRP), both of which cause the efflux of KP-1, is repressed in human pluripotent stem cells. Although KP-1, like other pluripotent markers, is not absolutely specific for pluripotent stem cells, the identified chemical probe may be used in conjunction with other reagents.
- Keywords
- FUNCTIONAL EXPRESSION; MULTIDRUG TRANSPORTER; HUMAN FIBROBLASTS; ABC TRANSPORTERS; GENE-EXPRESSION; SMALL-MOLECULE; GERM-CELLS; IN-VITRO; ES CELLS; DIFFERENTIATION
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50297
- DOI
- 10.1016/j.celrep.2014.02.006
- ISSN
- 2211-1247
- Article Type
- Article
- Citation
- CELL REPORTS, vol. 6, no. 6, page. 1165 - 1174, 2014-03
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