AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking
SCIE
SCOPUS
- Title
- AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking
- Authors
- Kwon, Yun; Shen, Jinbo; Lee, Myoung Hui; Geem, Kyoung Rok; Jiang, Liwen; Hwang, Inhwan
- Date Issued
- 2018-02
- Publisher
- National Academy of Sciences
- Abstract
- Protein trafficking is a fundamental mechanism of subcellular organization and contributes to organellar biogenesis. AtCAP2 is an Arabidopsis homolog of the Mesembryanthemum crystallinum calcium-dependent protein kinase 1 adaptor protein 2 (McCAP2), a member of the syntaxin superfamily. Here, we show that AtCAP2 plays an important role in the conversion to the lytic vacuole (LV) during early plant development. The AtCAP2 loss-of-function mutant atcap2-1 displayed delays in protein storage vacuole (PSV) protein degradation, PSV fusion, LV acidification, and biosynthesis of several vacuolar proteins during germination. At the mature stage, atcap2-1 plants accumulated vacuolar proteins in the pre-vacuolar compartment (PVC) instead of the LV. In wild-type plants, AtCAP2 localizes to the PVC as a peripheral membrane protein and in the PVC compartment recruits glyceraldehyde-3-phosphate dehydrogenase C2 (GAPC2) to the PVC. We propose that AtCAP2 contributes to LV biogenesis during early plant development by supporting the trafficking of specific proteins involved in the PSV-to-LV transition and LV acidification during early stages of plant development.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/50954
- DOI
- 10.1073/pnas.1717204115
- ISSN
- 0027-8424
- Article Type
- Article
- Citation
- Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 7, page. E1675 - E1683, 2018-02
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