ChIP-seq and RNA-seq methods to study circadian control of transcription in mammals.
SCIE
SCOPUS
- Title
- ChIP-seq and RNA-seq methods to study circadian control of transcription in mammals.
- Authors
- KIM, TAE KYUNG
- Date Issued
- 2015-01
- Publisher
- ELSEVIER ACADEMIC PRESS INC
- Abstract
- Genome-wide analyses have revolutionized our ability to study the transcriptional regulation of circadian rhythms. The advent of next-generation sequencing methods has facilitated the use of two such technologies, ChIP-seq and RNA-seq. In this chapter, we describe detailed methods and protocols for these two techniques, with emphasis on their usage in circadian rhythm experiments in the mouse liver, a major target organ of the circadian clock system. Critical factors for these methods are highlighted and issues arising with time series samples for ChIP-seq and RNA-seq are discussed. Finally, detailed protocols for library preparation suitable for Illumina sequencing platforms are presented.
- URI
- https://oasis.postech.ac.kr/handle/2014.oak/94313
- DOI
- 10.1016/bs.mie.2014.10.059
- ISSN
- 0076-6879
- Article Type
- Article
- Citation
- METHODS IN ENZYMOLOGY, vol. 551, page. 285 - 321, 2015-01
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.