Open Access System for Information Sharing

Login Library

 

Article
Cited 60 time in webofscience Cited 63 time in scopus
Metadata Downloads

SET7/9 methylation of the pluripotency factor LIN28A is a nucleolar localization mechanism that blocks let-7 biogenesis in human ESCs. SCIE SCOPUS

Title
SET7/9 methylation of the pluripotency factor LIN28A is a nucleolar localization mechanism that blocks let-7 biogenesis in human ESCs.
Authors
KIM, TAE KYUNG
Date Issued
2014-12-04
Publisher
CELL PRESS
Abstract
LIN28-mediated processing of the microRNA (miRNA) let-7 has emerged as a multilevel program that controls self-renewal in embryonic stem cells. LIN28A is believed to act primarily in the cytoplasm together with TUT4/7 to prevent final maturation of let-7 by Dicer, whereas LIN28B has been suggested to preferentially act on nuclear processing of let-7. Here, we find that SET7/9 monomethylation in a putative nucleolar localization region of LIN28A increases its nuclear retention and protein stability. In the nucleoli of human embryonic stem cells, methylated LIN28A sequesters pri-let-7 and blocks its processing independently of TUT4/7. The nuclear form of LIN28A regulates transcriptional changes in MYC-pathway targets, thereby maintaining stemness programs and inhibiting expression of early lineage-specific markers. These findings provide insight into the molecular mechanism underlying the post-translational methylation of nuclear LIN28A and its ability to modulate pluripotency by repressing let-7 miRNA expression in human embryonic stem cells.
URI
https://oasis.postech.ac.kr/handle/2014.oak/94315
DOI
10.1016/j.stem.2014.10.016
ISSN
1934-5909
Article Type
Article
Citation
Cell Stem Cell, vol. 15, no. 6, page. 735 - 749, 2014-12-04
Files in This Item:
There are no files associated with this item.

qr_code

  • mendeley

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher

김태경KIM, TAE KYUNG
Dept of Life Sciences
Read more

Views & Downloads

Browse